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A further DNA-based approach has long been to combine allergen with bacterial DNA sequences that include CpG motifs to selectively target Toll-like receptor 9 (TLR9) expressed on human B cells and dendritic cells. The main ragweed pollen allergen Amb a one covalently associated with a B-sort CpG-made up of oligodeoxynucleotide (ODN)131 was thriving